Jaringan Laboratorium Medis

Vol. 6 No. 2 (2024): November 2024
Original Articles

Quality Test of Soil Transmitted Helminth Eggs Using Natural Dye Extracted from Teak Leaves (Tectona grandis) at Concentrations of 40%, 60%, 80%, and 100% as an Alternative Dye in the Sedimentation Method

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  • Maysita Ari Anggraini,
  • Dina Afrianti
Maysita Ari Anggraini
Poltekkes Kemenkes Semarang
Dina Afrianti
Poltekkes Kemenkes Semarang

Published 2024-11-01

Keywords

  • Teak Leaf Juice,
  • STH (Soil Transmitted Helminth),
  • Eosin

Copyright (c) 2024 Jaringan Laboratorium Medis

Creative Commons License

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

How to Cite

Quality Test of Soil Transmitted Helminth Eggs Using Natural Dye Extracted from Teak Leaves (Tectona grandis) at Concentrations of 40%, 60%, 80%, and 100% as an Alternative Dye in the Sedimentation Method. (2024). Jaringan Laboratorium Medis, 6(2), 86-92. https://doi.org/10.31983/jlm.v6i2.11625

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Abstract

Helminthiasis is a parasitic infection in the form of worm larvae or eggs that develop in the human body. Diagnosing helminthiasis with fecal examination uses 2% eosin dye to clarify and emphasize the shape of worm eggs. Eosin 2% is a class-3 IARC carcinogenic reagent, so a natural reagent that does not damage the environment is needed. Reagents from natural materials that can be utilized are teak leaves containing anthocyanin compounds that can clarify and emphasize the shape of worm eggs.  The purpose of this study was to determine the quality of sth (soil transmitted helminth) worm eggs using natural dye teak leaf juice (tectona grandis) with a concentration of 40%, 60%, 80%, 100% as an alternative dye sedimentation method. The design of this study is quantitative with experimental design with posttest with control group design with Kruskal-Walis test. The results of this study obtained the concentration with the highest average value, namely 40% concentration of 8.7. The higher the concentration, the less clear the morphology of the egg because of the influence of dirt / residue from the juice. The results of the Kruskal-Walis test sig value 0.054, it can be concluded that there is no difference between teak leaf juice with a concentration of 40%, 60%, 80%, and 100% with 2% eosin dye as a control. Based on the mean rank value of the Kruskal-Walis test, it can be seen that the best teak leaf juice concentration is 40% concentration with a value of 34.50. This research can be used as a reference for the utilization of teak leaves as a natural dye to replace 2% eosin for STH worm eggs.
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References

  1. A. Arifta, H. R., Putera Makkadafi, S., Kemenkes Kalimantan Timur, P., & Islam Sultan Aji Muhammad Idris Samarinda, U. (2022). Studi Deskriptif Pemeriksaan Efektifitas Sampel Feses Metode Langsung dan Sedimentasi Telur STH (Soil Transmitted Helminth). Borneo Journal of Science and Mathematics Education BJSME: Borneo Journal of Science and Mathematics Education, 2(3), 2022. Diakses pada 29 September 2023. https://journal.uinsi.ac.id/index.php/bjsme/article/view/5916/1987.
  2. Andayani, I. G. A. S., Sulastri, S., Hananto, D. A., & Sriasih Made. (2020). Ekstrak Daun Jati (Tectona grandis) Alternatif Pewarna Pada Perhitungan Jumlah dan Viabilitas Sel Kultur Dibandingkan Dengan Pewarna Tryphan Blue. Jurnal Ilmiah Biologi , 8. Diakses pada 30 September 2023. https://ejournal.undikma.ac.id/index.php/bioscientist/article/view/3015/2201
  3. Angria, N. K. (2023). Edukasi dan Pemeriksaan Kecacingan pada Anak SDN Baddoka Kota Makassar. Jurnal Visi Pengabdian Kepada Masyarakat, 04, 50–58. Diakses pada 24 September 2023. https://ejournal.uhn.ac.id/index.php/pengabdian/article/view/1332/823.
  4. Apsari, P. I. B., Winianti, N. I., Arwati, H., & Dachlan, Y. P. (2020). Gambaran Infeksi Soil Transmitted Helminth Pada Petani di Desa Gel Gel Kabupaten Klungkung. Jurnal Lingkungan & Pembangunan, 4(2). Diakses pada 28 September 2023. https://ejournal.warmadewa.ac.id/index.php/wicaksana.
  5. Bedah, S., & Syafitri, A. (2018). Infeksi Kecacingan pada Anak Usia 8-14 Tahun di Rw. 007 Tanjung Lengkong Kelurahan Bidaracina, Jatinegara, Jakarta Timur. In Open Journal System (OJS): journal.thamrin.ac.id (Vol. 10, Issue 1). Diakses pada 24 September 2023. http://journal.thamrin.ac.id/index.php/JIK/article/view/106.
  6. Fathinatullabibah, Kawiji, & Umi Khasanah Lia. (2014). Stabilitas Antosianin Ekstrak Daun Jati (Tectona grandis) terhadap Perlakuan pH dan Suhu. Jurnal Aplikasi Teknologi Pangan. Diakses pada 9 Mei 2024.
  7. Fauzia, E. T., Majidah, L., & Prasetyaningati, D. (2019). Identifikasi Telur Cacing Ascaris Lumbricoides Pada Siswa Kelas 3 Sekolah Dasar Negeri Badas Desa Badas Kecamatan Sumobito Kabupaten Jombang. In Jurnal Insan Cendekia (Vol. 6, Issue 2). Diakses pada 30 September 2023. https://digilib.itskesicme.ac.id/ojs/index.php/jic/article/view/541.
  8. Fatarani Nadhira, F., Rahmat, M., Sundara Mulia, Y., & Rismiarti, Z. (2023). Ekstrak Daun Jati (Tectona grandis) Sebagai Alternatif Pengganti Eosin Dalam Pemeriksaan Telur Cacing Golongan Soil Transmitted Helminths. Jurnal Kesehatan Siliwangi, 4(1), 165–171. https://doi.org/10.34011/jks.v4i1.1502
  9. Hastuti, P., & Haryatmi, D. (2021). Efektivitas Rendaman Daun Jati (Tectona grandis Linn.f) Dalam Mewarnai Stadium Telur Parasit STH (Soil Transmitted Helminth). In Journal of Pharmacy (Vol. 10, Issue 2). Diakses pada 19 Agustus 2023. https://www.researchgate.net/publication/356926045_Efektivitas_Rendaman_Daun_Jati_Tectona_grandis_Linnf_Dalam_Mewarnai_Stadium_Telur_Parasit_STH_Soil_Transmitted_Helminth.
  10. Kementerian Kesehatan Republik Indonesia. (2023, January). Kemenkes Minta Masyarakat Untuk Waspadai Sejumlah Penyakit Tropis Ini. Diakses pada 19 Agustus 2023. http://p2p.kemkes.go.id/kemenkes-minta-masyarakat-untuk-waspadi-sejumlah-penyakit-tropis-ini/#:~:text=Angka%20prevalensi%20kusta%20di%20Indonesia,03%20kasus%20per%20100.000%20penduduk
  11. Kusumawati, L., Pratiwi, D., Wardani, K., Mujahid, I., & Almanfaluthi, M. L. (2022). Perbandingan Variasi Konsentrasi dan Waktu…(Lindah Kusumawati dkk) Perbandingan Variasi Konsentrasi dan Waktu Perendaman Ekstrak Batang Pohon Jati Sebagai Pewarna Alternatif Eosin 2% pada Pemeriksaan Telur Soil Transmitted Helminth The Comparison Of Concentration Variations and Soaking Time Of Teak Tree Trunk Extract As an Alternative Dye Of 2% Eosin on Soil-Transmitted Helminth Eggs Examination. Jurnal Biotek Medisiana Indonesia No.11 Vol. 2. Hal 123-132. Diakses pada 21 Agustus 2023.
  12. Lailatusyifa, N., Sartika, R. A. D., & Nuryati, T. (2022). Determinan Kejadian Kecacingan pada Siswa SD. Jurnal Ilmu Kesehatan Masyarakat, 11(01), 57–67. Diakses pada 24 September 2023. https://doi.org/10.33221/jikm.v11i01.1007.
  13. Miftahul, O., Rafhani, M., & Diterbitkan, R. (2020). Buku Ajar Mata Kuliah Statistika “Aplikasi di Dunia Kesehatan.”
  14. Oktari, A., & Mu’tamir, A. (2017). Optimasi Air Perasan Buah Merah (pandanus sp.) Pada Pemeriksaan Telur Cacing. Www.Teknolabjournal.Com), 6(1), 8–17. Diakses pada 25 November 2023. https://www.teknolabjournal.com/index.php/Jtl/article/view/85.
  15. Salnus, S., Dzikra Arwie, & Zulfian Armah. (2021). Ekstrak Antosianin Dari Ubi Ungu (Ipomoea Batatas L.) Sebagai Pewarna Alami Pada Pemeriksaan Soil Transmitted Helminths (STH) Metode Natif (Direct Slide). Jurnal Kesehatan Panrita Husada, 6(2), 188–194. Diakses pada 19 Agustus 2023. https://doi.org/10.37362/jkph.v6i2.649.
  16. Suraini, & Sophia, A. (2022). BIOMA : Jurnal Biologi Makassar Optimasi Air Perasan Ubi Jalar Ungu Ipomea batatas L. Pada Pemeriksaan Telur Cacing. Diakses pada 19 Agustus 2023. https://journal.unhas.ac.id/index.php/bioma.
  17. Surianti, Husain Halimah, & Sulfikar. (2019). Uji Stabilitas Pigmen Merah Antosianin Dari Daun Jati Muda (Tectona grandis Linn f) terhadap pH sebagai Pewarna Alami. Jurnal Chemica. Diakses pada 5 Mei 2024. https://jatp.ift.or.id/index.php/jatp/article/view/79.
  18. Winastri, N. L. A. P., Muliasar, H., & Hidayati, E. (2020). Aktivitas Antibakteri Air Perasan Dan Rebusan Daun Calincing (Oxalis Corniculata L.) Terhadap Streptococcus Mutans. In Agustus (Vol. 19, Issue 2). Diakses pada 1 November 2023. https://e-journal.biologi.lipi.go.id/index.php/berita_biologi/article/view/3786/pdf.