Jaringan Laboratorium Medis

Vol. 7 No. 1 (2025): May 2025
Original Articles

The Effect of EDTA Blood Sample Stability on Hemoglobin and Hematocrit Examination in Kedung II Public Health Center Employees Using an Automatic Method

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  • Anisa Ayu Anjani,
  • Dewi Hartinah,
  • Shinta Dwi Kurnia,
  • Yunita Rusidah,
  • Arief Adi Saputro
Anisa Ayu Anjani
Universitas Muhammadiyah Kudus
Shinta Dwi Kurnia
Universitas Muhammadiyah Kudus
Yunita Rusidah
Universitas Muhammadiyah Kudus
Arief Adi Saputro
Universitas Muhammadiyah Kudus

Published 2025-05-01

Keywords

  • Blood sample stability,
  • EDTA,
  • Hemoglobin,
  • HHematocrit,
  • Sysmex XP-100

Copyright (c) 2025 Jaringan Laboratorium Medis

Creative Commons License

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How to Cite

The Effect of EDTA Blood Sample Stability on Hemoglobin and Hematocrit Examination in Kedung II Public Health Center Employees Using an Automatic Method. (2025). Jaringan Laboratorium Medis, 7(1), 76-85. https://doi.org/10.31983/hezj1259

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Abstract

This study aims to determine the effect of EDTA blood sample stability on hemoglobin (Hb) and hematocrit (Ht) examination results using an automatic method. EDTA blood samples from 38 employees of Kedung II Public Health Center were examined immediately (0 hours), after a 1-hour delay at room temperature (17–210C), and after a 1-hour delay in a refrigerator (2–80C). The results were analyzed using a Two-Way ANOVA test. The study showed no significant differences in hemoglobin and hematocrit levels between the three storage conditions (ρ > 0.05). The average hemoglobin levels were 12.834 g/dL at 0 hours, 12.797 g/dL after 1 hour at room temperature, and 12.829 g/dL after 1 hour in the refrigerator. The average hematocrit levels were 38.484% at 0 hours, 38.321% after 1 hour at room temperature, and 38.434% after 1 hour in the refrigerator. The conclusion of this study is that storing EDTA blood samples for 1 hour, whether at room temperature or in the refrigerator, does not significantly affect hemoglobin and hematocrit examination results.

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